Liquid BioOne FAQs

bioone septic tank drain maintenance

  1. What is Liquid BioOne?
    Liquid BioOne’s formulation is patented and therefore unique in the industry. It contains no added enzymes, relying on the powerful action of its performance-ready, live vegetative microbes to degrade and digest fats, oil, grease, and other stubborn organic wastes to harmless by-products and water. The microbes in liquid BioOne are formulated to withstand and flourish even in the harsh conditions of commercial kitchens, making it the ideal bioproduct for grease trap maintenance. Liquid BioOne is performance-ready – NO MIXING and NO WAITING required – so residential and commercial customers routinely use more product with greater results. Liquid BioOne is the premier drain line product in the country.
  2. What is the difference between BioOne and a de-greasing product?
    De-greasing products are emulsifiers and do just what they say, move grease. When you apply a de-greasing product, the grease is liquefied and then moved down the pipe where it will again solidify. De-greasing products do work in front of your eyes but simply move the grease to another location. BioOne is a bacteria that will actually consume the fats, oils and grease in the pipe and digest it, leaving nothing but carbon dioxide and water. BioOne eliminates grease build up completely instead of relocating it.
  3. Do the live bacteria in BioOne die off in the bottle? How do the bacteria survive without consuming each other?
    Aaahhh…the beauty of a patent! Liquid stabilization of BioOne’s microorganisms is just one of the patents that protect the formulation. But to understand what this stabilization process does, compare it to the hibernation of a grizzly bear. The bear’s metabolic functioning slows down dramatically to the point that no food is needed. When the resting state is ended, that bear is ready for an all-you-can-eat buffet. In the case of liquid BioOne, when you dose product into a wastewater system, the live microbes are immediately ready to eat, digest, and consume grease, oil, fat, and stubborn organic matter found in every drain pipe, septic system, and grease trap.
  4. Does Liquid BioOne freeze? Does freezing affect the effectiveness of BioOne’s microorganisms?
    Like PVC glues and other liquid products carried on service trucks, liquid BioOne will freeze but freezing in no way affects the viability and effectiveness of the microbial activity. In fact, the microbes in liquid BioOne are actually freeze-dried before being formulated into the liquid state.
  5. Does Liquid BioOne degrade hair?
    BioOne does not degrade hair, but rather consumes the fats, oil, grease and organic matter which bind the hair in the pipe. Once the buildup is removed the hair moves freely through the line.
  6. Does Liquid BioOne have a scent?
    There are no perfumes in liquid BioOne; it has a natural, earthy scent.
  7. How long is Liquid BioOne’s shelf life?
    Although the microbes in liquid BioOne stay viable for years, peak product effectiveness is 18 months. Naturally, shelf life is insignificant because BioOne users don’t hold on to inventory that long. End users find liquid BioOne so easy to use that they regularly consume more product on a routine basis—keeping their drain line systems free of organic buildup.
  8. How many applications are in the residential 64oz. bottle?
    There are 32, two-ounce applications in each 64oz. residential-size bottle of liquid BioOne.
  9. How does liquid BioOne perform in commercial kitchen drain lines and grease traps?
    Liquid BioOne is specially formulated to adapt to the harsh conditions of commercial kitchen drain lines and grease traps. There is no down time with liquid BioOne; the performance-ready activity of BioOne means every minute the product is in the system, grease, oil, and fats, as well as other stubborn organic matter, are being eaten, digested, and consumed. The exclusive liquid BioOne Auto Dispensing System (ADS) is ideal for all commercial kitchen environments, dosing BioOne at set intervals into the main line directly leading into the grease trap – providing the customer with 24/7 protection. Typically a trap treated with liquid BioOne can see a normal reduction of necessary pumping by as much as 50%.
  10. What temperature range can the microbes in liquid BioOne withstand?
    Liquid BioOne’s microbes can survive in temperatures as high as 105 degrees, the normal operational range found in commercial kitchens. When wastewater enters the system that exceeds this temperature, many of the organisms will survive because liquid BioOne forms a biofilm around the entire surface of the pipe. High water temperatures and chemicals flowing through the drain line do not fill the entire pipe but rather flow along the middle and bottom of the line. In the commercial kitchen setting the liquid BioOne auto-dispensing system provides for the normal turnover rate of microbes in these types of settings by continuously replenishing a fresh supply.
  11. What pH range do the microbes in liquid BioOne require, and is it necessary to add a pH neutralizer to a grease trap or septic system before dosing with liquid BioOne?
    When using liquid BioOne it is unnecessary to pretreat a septic or grease trap system with pH neutralizer. Liquid BioOne is formulated to perform in very harsh environments, including lower or higher pH ranges. Liquid BioOne’s microbes will degrade fats, oils, grease, and organic waste in a pH range of 5-9.
  12. Is Liquid BioOne in compliance with national and local ordinances?
    Yes. A big concern that municipalities have is liquefying or emulsifying fats, oils, and greases that simply push the problem down the line to the city. Liquid BioOne contains NO ADDED ENZYMES OR SOAPS. Enzymes change the physical state of a compacted solid buildup to a loose solid allowing grease and organic matter to flow directly to the wastewater treatment plant where it has to be biotreated before it can be reintroduced into the environment. Soaps emulsify fats, oils, and greases. Emulsified grease and oils require bioremediation at the municipality, and that’s very expensive. That expense is often transferred back to the commercial customer in the form of surcharges.
    Liquid BioOne eats, digests, and consumes grease, oil, fats, and organic matter by degrading it into harmless carbon dioxide and water. Most municipalities across the country will allow liquid BioOne to be introduced into commercial wastewater systems. Offering liquid BioOne to your commercial customers will allow them to avoid potentially expensive wastewater surcharges.

Commercial Lift Station Before & After Treatment

Lift Station Before Treatment

lift station before treatment
Day 1 of Treatment at a lift station outside of Bradenton, FL located outside of a food court of a major shopping mall. On day 1 BioOne® was added via an AIS System and the perimeter was sprayed with BioOne.

Lift Station After Treatment

lift station after treatment
Day 30 of Treatment

Commercial Markets for the BioOne Auto-Dispensing System


  • On-site Wastewater Systems
  • Quick-Service Restaurants
  • Hotels and Motels
  • Family Restaurants
  • Specialty Bakeries
  • Nursing Homes
  • Schools



  • Hospitals
  • Jails and Prisons
  • MRI Facilities
  • Medical Labs
  • Photo Labs



  • Apartment and Condominium Complexes
  • Auto Garages
  • Cruise Ships
  • Life Stations
  • Supermarkets
  • Airports
  • Malls
  • Movie Theaters
  • Exhibition Halls
  • RV and Trailer Parks

Commercial Restaurant Problems from Fat, Oil and Grease (FOG)

fats and oils

Backed Up Drains

  • Expensive Slip and Falls
  • Potential Worker’s Compensation Claims
  • Emergency Pumping
  • Health Code Violations
  • Business Interruptions
  • Unsanitary Conditions

Offensive Odors

  • Unappetizing
  • Decreased Customer Traffic
  • Bad Advertising
  • Poor Work Environment

Drain Flies

  • Gives Impression of Poor Housekeeping and Lack of Hygiene
  • Annoying to Customers

Municipal Fines and Charges


Residential Drain Field Rejuvenation

drain fieldResidential drain fields are designed to allow the discharged water to percolate through the biomat. Drain fields fail for several reasons:

  1. Hydraulic overload (too much water being released into the septic tank and then discharged into the drain field)
  2. Organic overload (too much non-degraded grease, oil, and organic matter)
  3. Chemical overload (excessive chemicals destroy the bacteria necessary for degrading waste)
  4. Faulty design or installation

For best results, pump septic tank and add 2.5 gallons of liquid BioOne directly into the septic tank. Adding another 2.5 gallons of liquid BioOne for every 400 square feet of drain field through the distribution box and or header pipe is also strongly advised. Continue treatment by following the recommended dosing parameters.

commercial drain and grease trap maintenancebioone septic tank drain maintenance

For completely failed drain fields, the most immediate results will be obtained when liquid BioOne dosing is coupled with soil fracturing.

BioOne and Septic Systems

drain field septic systemSeptic systems require live bacteria to consume, digest, and degrade grease, oil, and other organic matter so that proper functioning can be maintained. When a septic system is properly maintained, the connected drain field can also function according to design.

Subjected to bleaches, detergents, and other chemicals, naturally occurring bacteria struggle to survive and keep up with the influx of waste in residential and commercial septic systems.

Liquid BioOne is formulated to work in the harsh conditions of septic systems. BioOne requires no pH neutralizing and is performance ready. BioOne contains no added enzymes or other emulsifying agents which only liquefy solid waste. BioOne’s bacteria eat and digest the solid waste without the unbalanced action of enzymes or surfactants.

The most effective way to maintain a septic system is to inoculate the tank with BioOne immediately after pumping.

After your septic system has been pumped: Your technician will add BioOne directly into the clean tank.

To properly maintain your septic system: Between pumping, follow the instructions on the label of the 64 oz. BioOne that your technician has left with you.

The Meaning of the EPA Safer Choice Program Logo

EPA SaferChoice logoWith 85% of businesses creating sustainability initiatives, and consumers showing increased interest in environmental matters, environmental conscious products are increasingly in demand. In fact, surveys show that a product’s environmental impact is an important factor considered by   consumers in making their buying decisions.

The U.S. EPA Safer Choice Program parallels worldwide interest in environmental safety and sustainability. The purpose of this program is to help identify and develop environmentally safer products.

Once a product passes the specifications of that program, the EPA allows the display of the Safer Choice logo. This logo “allows consumers to quickly identify and choose products that can help protect the environment and are safer for families.”

In order to pass EPA Safer Choice requirements, a product must undergo a rigorous review by the EPA and a third party scientific review team. This team carefully screens each ingredient for any potential human health and environmental effects. The team reviews not only the ingredient, but its chemical structure, to see how it could impact the environment and people.

Only products that are made of the safest possible ingredients are eligible for approval. BioOne has passed the U.S. EPA’s Safer Choice Program’s criteria and proudly displays the Safer Choice logo in recognition of its positive effect on human health and the environment.